Blog Date 29 March, 2023

Stable Isotope Labelled Compounds in Metabolism and Analytical tools

Stable Isotope Labelled Compounds in Metabolism and Analytical tools

Application of Stable isotope Labelled Compounds

Stable Isotope Labelled analogues can play a very important role in identifying metabolites in complex biological samples. Combined with the powerful analytical capabilities of mass spectrometry and NMR, they provide us with a very useful tool in understanding the metabolic pathways and biochemical

Mechanisms that lead to the formation of metabolites. Compounds labeled with stable isotopes, such as drug metabolism and toxicologists to gain better understanding of drugs’ disposition and their potential role in target organ toxicities have used deuterium and carbon-13, effectively.

Analytical Application of Stable isotope Compounds

Stable isotopes such as deuterium and carbon-13 can be incorporated strategically into drug molecules to facilitate the elucidation of their metabolites’ structures using LC/MS and NMR.

The combination of stable isotope-labeling techniques with mass spectrometry and nuclear magnetic resonance (NMR) spectroscopy allows rapid acquisition and interpretation of data, has promoted greater use of stable isotope-labeled compounds in absorption, distribution, metabolism, and excretion (ADME) studies.

With the availability of stable isotope-labeled analogues and robust LC/MS instruments, it has become straightforward to quantitate analytes in complex biological mixture.

Use of Stable Isotope Labeled Compounds in Locating Metabolites in Biological Fluids

The presence of twin ions in the mass spectra can greatly assist in the identification of metabolites in biological samples such as urine, bile, and plasma.

 Significance of Using Deuterated Solvents

  • The use of deuterium-labeled solvents to assist in the identification of metabolites.
  • In the presence of a deuterated solvent (such as D2O), the protons on functional groups such as OH, NH2, or SH will be exchanged with deuterium. This leads to mass shifts corresponding to the number of exchangeable protons in the molecule.


SimSon Pharma Limited is a reputed for doing accurate bio-analysis for its customers. In case you require any more information on stable isotope analysis, do let us know. We have a full-fledged facility with technicians for testing.

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